Supernatural tells the story of Sam Winchester (Jared Padalecki) and Dean Winchester (Jensen Ackles), two brothers who fight creatures that most people don't believe exist. The brothers lost their mother as children in a fire with supernatural elements. As a result, their father devoted his life to finding out what killed his wife, and seeking revenge. He brought his sons on the road with him, training them to fight evil. Sam left this life as a hunter in order to attend college at Stanford University, while Dean stayed with their father and helped with demon hunting. When their father suddenly disappears, Dean seeks Sam's assistance in finding him, and the brothers travel around the country in a black 1967 Chevrolet Impala paranormal events and other unexplained occurrences, many of them based on folklore, myths, and American urban legends, and others based on classic tales of supernatural creatures, such as vampires, werewolves, and ghosts.
Dean (Jensen Ackles) was born on January 24, 1979 and is the eldest of the two brothers. Dean drives a black four-door 1967 Chevy Impala (given to him by his father), and is a fan of classic rock music and heavy metal. He tends to make light of some of his and Sam's adventures, and is known to use crude humor and make sexual innuendos. Dean is terrified of flying, and claims that it is the reason why he drives everywhere. Despite his working knowledge of existence beyond death, he is skeptical towards religion. He also wears a metal amulet, given to him as a gift by his brother, on a long black cloth band necklace around his neck.
Sam (Jared Padalecki) was born on May 2, 1983 and is 4 years younger than Dean. He was a senior at Stanford before here rejoined his brother to go hunting again. Sam was only six months old when his mother died in his nursery and Dean carrued him out to safety. He is the brainiest out of the two. He also studies hard via books, radio, TV & internet with newspapers & walkie-talkies & a mobile phone.
Bobby (Jim Beaver) is a hunter like the Winchesters, and provides hunting and research support to the brothers, while running an auto salvage yard during the day. He became a hunter after being forced to kill his possessed wife. Bobby has amassed an extensive collection of resources about the supernatural, including literature and relics. Sometime before the events of the show, Bobby forged an extremely strong friendship with John Winchester. His relationship with Sam and Dean has developed to the point of family, with him acting as a father figure.
Castiel (Misha Collins) is an angel who helps Dean and his brother Sam in their battles with various demons and angels. He possesses a number of superhuman abilities, including the ability to exorcise demons with his touch. Initially, Castiel demonstrates little emotion, though his interactions with Dean have humanized him to a degree.
John (Jeffrey Dean Morgan) is the father of Dean and Sam Winchester. He is also a former Marine (Echo 2/1). John watched as his wife, Mary, was killed by Azazel in Sam's nursery on November 2, 1983. He raised his two sons to fight the supernatural, with help from hunters he met on his travels, including several customers of Harvelle's Roadhouse.
Mary (Samantha Smith) is the mother of Dean and Sam Winchester. Six months after Sam's birth, Mary was killed after discovering an intruder in Sams nursery and her husband John saw her in flames on the ceiling. It was after this happened that John and his two sons became hunters to avenge her death.
Jo (Alona Tal) is the daughter of Ellen and William Anthony Harvelle, the latter of which was a hunter who was killed during a hunt with John Winchester when Jo was still a child. She therefore treasures her father's pure iron knife engraved with his initials. Jo first meets Sam and Dean when they come looking for Ellen.
Ellen (Samantha Ferris) is the wife of hunter William Anthony Harvelle and the mother of Jo. She knew John Winchester. Ellen runs Harvelle’s Roadhouse, a saloon & pub frequented by hunters.
A demon. She first appeared in the third season, rescuing Sam and Dean Winchester, and continued to assist them. While Sam wanted to give her a chance, Dean remained distrustful of her.
'Katie Cassidy as Ruby (left) and Genevieve Cortese as Ruby (right)'